diffuse reflectance standard puck (20% spectralon)  (Labsphere Inc)

Journal: Journal of Biomedical Optics

Article Title: Empirical method for rapid quantification of intrinsic fluorescence signals of key metabolic probes from optical spectra measured on tissue-mimicking turbid medium

doi: 10.1117/1.JBO.26.4.045001

Figure Lengend Snippet: The empirical model with a pair of single wavelengths for accurate attenuation removal in 2-NBDG phantoms. λ ex = 450 nm , λ em = 550 nm , the parameter set [ α , β ] = [ 5.96 , − 6.44 ] . (a) Optical properties of phantoms; (b) diffuse reflectance spectra; (c) raw fluorescence; and (d) corrected fluorescence of phantoms with fixed reduced scatterings but different absorption coefficients (top) and fixed absorption but different reduced scatterings (bottom).

Article Snippet: After all optical measurements on the phantoms were completed, reference spectra on a diffuse reflectance standard puck (20%, Spectralon, Labsphere) and a fluorescence standard puck (USF210-010, LabSphere) were collected for future calibration.

Techniques: Fluorescence

Journal: Journal of Biomedical Optics

Article Title: Empirical method for rapid quantification of intrinsic fluorescence signals of key metabolic probes from optical spectra measured on tissue-mimicking turbid medium

doi: 10.1117/1.JBO.26.4.045001

Figure Lengend Snippet: (a) The effect of CWL band of diffuse reflectance collection on the mean percent error of the empirical model for 2-NBDG extraction when bandwidth is fixed at 10 nm. (b) The corresponding powers [ α , β ] for the combination of different CWLs.

Article Snippet: After all optical measurements on the phantoms were completed, reference spectra on a diffuse reflectance standard puck (20%, Spectralon, Labsphere) and a fluorescence standard puck (USF210-010, LabSphere) were collected for future calibration.

Techniques: Extraction

Journal: Journal of Biomedical Optics

Article Title: Empirical method for rapid quantification of intrinsic fluorescence signals of key metabolic probes from optical spectra measured on tissue-mimicking turbid medium

doi: 10.1117/1.JBO.26.4.045001

Figure Lengend Snippet: The empirical model with a pair of single wavelengths for accurate attenuation correction in TMRE phantoms. λ ex = 550 nm , λ em = 580 nm , the parameter set [ α , β ] = [ − 10.25 , 10.58 ] . (a) Optical properties of phantoms; (b) diffuse reflectance spectra; (c) raw fluorescence; and (d) corrected fluorescence of phantoms with fixed reduced scatterings but different absorption coefficients (top) and fixed absorption but different reduced scatterings (bottom).

Article Snippet: After all optical measurements on the phantoms were completed, reference spectra on a diffuse reflectance standard puck (20%, Spectralon, Labsphere) and a fluorescence standard puck (USF210-010, LabSphere) were collected for future calibration.

Techniques: Fluorescence

Journal: Journal of Biomedical Optics

Article Title: Empirical method for rapid quantification of intrinsic fluorescence signals of key metabolic probes from optical spectra measured on tissue-mimicking turbid medium

doi: 10.1117/1.JBO.26.4.045001

Figure Lengend Snippet: (a) The effect of CWL band of diffuse reflectance filter on the mean percent error of the empirical model for TMRE extraction, the bandwidth is fixed at 10 nm. (b) The corresponding powers [ α , β ] for the combination of different CWLs.

Article Snippet: After all optical measurements on the phantoms were completed, reference spectra on a diffuse reflectance standard puck (20%, Spectralon, Labsphere) and a fluorescence standard puck (USF210-010, LabSphere) were collected for future calibration.

Techniques: Extraction